| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Stewart, Quinlan M (6)
|
MAWAP |
30.89 |
29.95 |
-0.94 |
| 2 |
Klaehn, Milla L (6)
|
MATRI |
26.72 |
29.96 |
3.24 |
| 3 |
Deveney, Brynn K (6)
|
MATRI |
33.86 |
34.34 |
0.48 |
| 4 |
Cooper, Lindsay A (6)
|
MATRI |
40.46 |
37.55 |
-2.91 |
| 5 |
Scott, Lucy M (6)
|
MAWAC |
|
39.31 |
- |
| 6 |
Bohn, Freya A (6)
|
MAWAP |
40.17 |
44.91 |
4.74 |
| 7 |
Jones, Madeleine H (6)
|
MAWAC |
1:02.62 |
51.57 |
-11.05 |
| 8 |
Jubb, Penelope E (6)
|
MAWAC |
|
52.06 |
- |
|
Reber, Peyton E (6)
|
MAFSSC |
36.73 |
DQ |
- |
|
Bijlani, Shaina J (6)
|
MAMSA |
46.03 |
DQ |
- |
|
Benevento, Saoirse L (6)
|
MAWAP |
|
DQ |
- |
|
Brost, Ruby A (6)
|
MAWWC |
|
DQ |
- |
|
Grafe, Elizabeth J (6)
|
MAWAC |
|
DQ |
- |
|
Eruslanova, Elizabeth (6)
|
MASSC |
|
NS |
- |